Laminins are a family of cell-type specific basement membrane proteins important for cell adhe-sion, differentiation, migration and phenotype stability. In his talk, Dr Sigmundsson will provide examples from several of his ongoing projects, involving culturing of primary progenitor cells from various tis-sues, applying specific cell culture matrices of recombinant laminins together with defined media and high content imaging. The aim of this activity is to define robust methods for establishment and expansion of pluripotent stem- and progenitor cells, suitable for regenerative medicine.